FEATURON - Calibration Certificate (ISO 8655)

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CALIBRATION CERTIFICATE

📋 Ref PSRF:

🔬 Select Pipette (S/N):

Customer Details:

Certificate No:
Received Date:
Calibration Date:
Recom Due Date:
Cert. Issued Date:
Calibrated at:

The piston operated device calibration carried out with respect to standard as per ISO 8655-6:2002 in compliance with National/International Standards.

Unit Under Calibration:

Description		Serial No
Make		ID no
Model		Calibration Points(µl)
Volume Range(µl)		Condition on Receipt
Least Count(µl)		Accuracy(µl)±
Location		No of Channel

Environmental conditions:

Temperature(°C)

Relative Humidity(%)

Air Pressure(hPa)

Standard's Traceability Details: ⚙ Standards & Traceability Master

Sl.No	Equipment / Reference Standard	Sl No	Calibrating Agency	Traceability No	Valid upto	Cert
1						📄
2						📄

Calibration Results (Mechanical Volumes):

Sl No	Device Readings(µl)	Calculated Mean Volume at 27 °C(µl)	Error(µl)	Uncertainty(±µl)
1	100	100.13	0.13	0.29
2	500	499.31	-0.69	0.75
3	1000	1000.66	0.66	1.23

As per ISO 8655-2:2002 systematic error, Random error and Error limits:

Sl.No	Device Readings(µl)	Calculated Mean Volume at 27 °C(µl)	Systematic Error(µl)	Syst Error Limits(±µl)	Random Error(µl)	Random Error Limits(±µl)
1	100	100.13	0.13	0.80	0.13	0.30
2	500	499.31	-0.69	4.00	1.11	1.50
3	1000	1000.66	0.66	8.00	1.90	3.00

Remarks:

* Calculated Mean Value given is the average of 10 measurements

* Equipment used for calibration were calibrated & Traceable to National and International Standard

* The reported Expanded Uncertainty is calculated at 95.45 % C.L. with coverage factor k=2

* This calibration certificate is valid for scientific & industrial purpose only

* Ref PSRF No:

* This report refers only to particular item submitted for calibration

Status: PASS (According to ISO 8655-2:2002 permissible limits)

Calibrated by

Calibration Engineer

Page No 1 of 2

Authorised Signatory by

QM / TM

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Annexure to Certificate No: 801

Calibration Date: --

CALIBRATION REPORT - RAW GRAVIMETRIC OBSERVATIONS (ANNEXURE)

Individual 10 Weighing Observations per Volume Target as per ISO 8655-6:2002 / ISO 8655:2022 Gravimetric Procedure

Customer: --

Instrument: --

Serial No: --

Range: --

Standard Balance: --

Z-Factor: µL/mg

Water Temp:

1. Minimum Volume: µL (Vmin)

Run	Mass m_i (mg)	Volume V_i (µL)
1		100.02
2		100.00
3		99.97
4		100.03
5		100.01
6		99.99
7		100.04
8		99.98
9		100.02
10		100.00

Target Volume V_s:100.00 µL

Mean Mass m̄:0.000 mg

Mean Volume V̄:0.00 µL

Systematic Error e_s:0.00 µL

Inaccuracy A%:0.00%

ISO Permissible Limit ±A%:±%

Standard Deviation s:0.00 µL

Random Error CV%:0.00%

ISO Permissible Limit CV%:≤%

Uncertainty U (k=2):±0.00 µL

Result:PASS

2. Middle Volume: µL (Nominal/2)

Run	Mass m_i (mg)	Volume V_i (µL)
1		500.05
2		500.00
3		499.94
4		500.08
5		500.02
6		499.97
7		500.03
8		499.98
9		500.02
10		500.01

Target Volume V_s:500.00 µL

Mean Mass m̄:0.000 mg

Mean Volume V̄:0.00 µL

Systematic Error e_s:0.00 µL

Inaccuracy A%:0.00%

ISO Permissible Limit ±A%:±%

Standard Deviation s:0.00 µL

Random Error CV%:0.00%

ISO Permissible Limit CV%:≤%

Uncertainty U (k=2):±0.00 µL

Result:PASS

3. Nominal Volume: µL (Nominal)

Run	Mass m_i (mg)	Volume V_i (µL)
1		1000.09
2		999.99
3		999.94
4		1000.04
5		1000.01
6		999.97
7		1000.03
8		999.98
9		1000.02
10		1000.00

Target Volume V_s:1000.00 µL

Mean Mass m̄:0.000 mg

Mean Volume V̄:0.00 µL

Systematic Error e_s:0.00 µL

Inaccuracy A%:0.00%

ISO Permissible Limit ±A%:±%

Standard Deviation s:0.00 µL

Random Error CV%:0.00%

ISO Permissible Limit CV%:≤%

Uncertainty U (k=2):±0.00 µL

Result:PASS

* Gravimetric Conversion: V_i = m_i × Z (Z-Factor temperature & pressure correction per ISO 8655-6).

* Systematic Error (Inaccuracy): e_s = V̄ - V_s; A% = (e_s / V_s) × 100%.

* Random Error (Imprecision): s = √[Σ(V_i - V̄)² / (n - 1)]; CV% = (s / V̄) × 100%.

* Expanded Uncertainty: U = k × √[(s / √n)² + u_bal²], with coverage factor k=2 (approx. 95.45% confidence interval).

Observed & Recorded by

Calibration Engineer

Page No 2 of 2

Verified & Approved by

Quality Manager / Technical Manager

QM / TM